Isolation of yeast strains
The samples were serially diluted, plated on a selective medium, Martins Rose
Bengal agar Medium (33.6 gm /liter) and were incubated at 28°C for 48 hours. The colonies
appeared were further purified. Pure colonies were isolated and tested for further
characterization.
Morphological characterization
The strains were stained by lactophenol-cotton blue, carbol fuchsin and seen under
phase contrast microscope. The colonies were checked for their colour, outline and other