When grazing is decreased in winter, significantly higher amounts of concentrated feed, especially corn silage and grass, are given to meet the energy needs of high-yielding cows. In the case of contaminated feed usage, AFM1 will be present in the milk for 2–3 days following ingestion (Prandini et al., 2009). Therefore, the risk is largely related to grain-based feed (Duarte et al., 2013 and Fink-Gremmels, 2008). The transmission of AFB1 from foodstuffs to milk (carry-over) in dairy cows is influenced by various nutritional and physiological factors, including feeding, the degree of digestion, animal health, biotransformation capacity of the liver, and milk production (Duarte et al., 2013).