Criteria for selection of extracted teeth for this study were:
1. Deciduous teeth, free from caries.
2. Deciduous teeth, free from any morphological defects. 3. Deciduous teeth with no previously placed restorations. 4. Deciduous teeth, free from cracks.
Each donor was asked to provide confidential histories by answering questions in a proforma which was kept in the tooth bank record. Parents of the patients undergoing extraction procedure for a variety of reasons were explained about the future use of these extracted teeth as biological restoration. The consent of donors was obtained on a duly signed consent form.
Methods
Tooth bank procedure
The collected samples of extracted teeth were thoroughly scaled, polished, and freed of soft tissues and periodontal remnants [Figure 1]. The pulps were removed from root canals and complete biological preparation was done [Figure 2]. After preparation, all the sample teeth were placed in the ultrasonic tank operating at 42 GHz and 100 W output, at five working cycles in 6% H 2 O 2 [Figure 3]. Each tooth was sonicated for 30
minutes. Teeth were stored at 4 o C in Hank's balanced salt solution (HBSS) with donor identification till the time it was used [Figure 4] and [Figure 5].