With nitrite reduction being considered the key step in denitrification to distinguish true denitrifiers from nitrate reducers ,only 1.96% of the isolates from this study were found to reduce bouth nitrate and nitrite. Out of the 14 DNB isolated and characterized for denitrification properties from brackishwater shimp culiyre ponds,9 isolates with considerable difference in their ability to denitrify were identified as Marinobacter spp.. While most of the isolates belonged to the order Alteromonadales, a few belonged to the order Rhizobiales and Actinomycetales, a few belonged to the order Rhizobiales and Actinomycetales. It should be noted that members of the family Alteromonadaceae and other Alteromonas-like proteobacteria usually do not denitrity except a few While marinobacter spp. Isolate from different ecosystems are reported to carry out denitrification some are reported to degrade hydrocarbons under denitrifying conditions. Though the occurrence of Marinobacter Hydrocarbonoclasticus in shrimp culture ponds has been reported using a culture independent approach based on the nosZ gene sequence similarity, efforts to isolate and characterize such denitrifying bacteria remains limited. Our study provides the proof of experimental verification of denitrification in Marinobacter spp..