3.4. Extractability of antioxidative compounds
Extractability factors describing the efficiency of the different
extraction system used in the study are presented in Table 5. Based
on EF values it may be supposed that antiradical and lipid preventing
compounds from control breads possess rather lipophilic
character, and QL supplemented them with hydrophilic active
compounds, a reverse situation was observed for CHEL, while
reductive compounds were more effectively extracted from all
samples using PBS buffer. As expected, LPO from control breads
were most effectively extracted using MeOH:H2O (1:1, v/v)
(FF ¼ 0.38), however QL addition enriched breads with bufferextractable
phytochemicals exhibiting this activity (FF values
about 1). Simulated digestion released multifaceted antioxidant
compounds from all samples much more effectively than chemical
extraction MeOH:H2O (1:1, v/v). The most important differences
were visible in the cases of reductive (EF values from 7.24 to 23.86)
and antiradical (EF values from 3.85 to 5.24) compounds, the lowest
for LPO (EF values from 1.15 to 3.16) and CHEL (1.73e3.75). The
same relationship was observed for AE. Regardless of used
methods, extractability of all antioxidants decreased with QL
addition higher than 3 g/100 g. Obtained data clearly illustrate
essential differences between both used procedures (chemical
extraction and simulated gastrointestinal tract action) and point
the need for standardization and harmonization of procedures for
assessing the activity of newly designed food products.
3.4. Extractability of antioxidative compoundsExtractability factors describing the efficiency of the differentextraction system used in the study are presented in Table 5. Basedon EF values it may be supposed that antiradical and lipid preventingcompounds from control breads possess rather lipophiliccharacter, and QL supplemented them with hydrophilic activecompounds, a reverse situation was observed for CHEL, whilereductive compounds were more effectively extracted from allsamples using PBS buffer. As expected, LPO from control breadswere most effectively extracted using MeOH:H2O (1:1, v/v)(FF ¼ 0.38), however QL addition enriched breads with bufferextractablephytochemicals exhibiting this activity (FF valuesabout 1). Simulated digestion released multifaceted antioxidantcompounds from all samples much more effectively than chemicalextraction MeOH:H2O (1:1, v/v). The most important differenceswere visible in the cases of reductive (EF values from 7.24 to 23.86)and antiradical (EF values from 3.85 to 5.24) compounds, the lowestfor LPO (EF values from 1.15 to 3.16) and CHEL (1.73e3.75). Thesame relationship was observed for AE. Regardless of usedmethods, extractability of all antioxidants decreased with QLaddition higher than 3 g/100 g. Obtained data clearly illustrateessential differences between both used procedures (chemicalextraction and simulated gastrointestinal tract action) and pointthe need for standardization and harmonization of procedures for
assessing the activity of newly designed food products.
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