ThePCR reactions consisted of 25 ul reaction volumes containing 1×PCR buffer with 0.2 mM of each of thedNTPs, 1.5 mM MgSO4, 0.2 uM of each of the specific forward and biotinylated reverse primers, 0.625 U of Platinum Taq DNA Polymerase High Fidelity, and 1 ul of the DNA sample.