In this study, conventional CTAB DNA extraction
method was used to isolate DNA from various types
of samples including animal feeds, raw materials and
processed foods. DNA extraction methods must be
efficient, yielding as much DNA as possible from the
sample matrix (Smith & Maxwell, 2007). The CTAB
DNA extraction method can extract pure DNA of the
high molecular weight from plants and is an efficient
method for a wide range of plant materials and plantderived
foods by providing a good separation of
DNA from polysaccharides (Jasbeer et al., 2008).
According to Mafra et al. (2008), the extraction
efficiency of the extraction protocol was evaluated
by the determination of yield and purity of DNA
extracts, as well as amplificability of DNA extracts.
Therefore in this study, the CTAB extraction method
was evaluated by looking from three different
aspects, which are DNA yield, DNA purity and DNA
amplification.