Data obtained from the experiments were expressed as
mean ± standard error of mean (SEM). For statistical
analysis, data were subjected to one-way analysis of
variance (ANOVA) followed by Student’s t- test. A level of
P < 0.05 was acceptable as significant unless otherwise
stated. GraphPad Prism version 5.0 for windows was used
for these statistical analyses (GraphPad software, San
Diego California USA).