Latex rubber sheet wastewater was collected from a lagoon
pond of a CRSF at the Phichit suburb in Songkhla Province,
Thailand. The wastewater was collected as a composite sample
at a deep level roughly 20–100 cm from the surface at various
positions of the pond for obtaining a representative sample
and pouring them into a 25 L plastic tank. The collected
wastewater was filtered using a cheesecloth to remove solid
particles, and directly used as the raw wastewater medium
without supplementing with any nutrients, and with no autoclaving
(RSW).