Though there were good levels of total BoMinD transcript in transgenic cauliflower (CM1–CM6) lines using RT-PCR with BoMinD primers, there was only weak BoMinD trans-gene expression detected in CM4, CM5 and CM6 lines with BoMinD For and E9-ter Rev primers (Fig. 1E). Examination of BoMinD levels by western blotting with anti-MinD antibody also showed very low BoMinD levels in stable BoMinD transgenic cauliflower lines(Fig. 1F).