The 20 fractions were
combined into four fractions, fractions B2A-B2D, based on silica
TLC (CHCl3-MeOH-H2O, 7:3:1) results. Fraction B2A was
subjected to silica gel CC with CHCl3-MeOH-H2O (40:10:1)
as eluent and purified by a Sephadex LH-20 column with
EtOH-H2O (6:4), respectively. Fraction B2A yielded three
glycosides