Additives act as growth factors to promote the tissue repair, migrating cell into the wound site and stimulating cell proliferation [32]. Initially, a study of using different additives concentration effect on the wound closure was conducted to determine its healing capability of NIH 3T3 cells. Based on the results (Table 1(a)), the response obtained from each NIH 3T3 cells treatment exposed to different concentrations of additives was distinguishable (p < 0.01) than responses received in the control group. In brief, the use of 50 ppm additives was noted to be insufficient to heal the wounds within 24 h relative to 100 ppm additives