DNA Fingerprinting Techniques. One of the most widely used culture-independent molecular techniques is T-RFLP, first developed in 1997 for studying the structure and
diversity of bacterial communities in the environment
(Dickie and Fitz John, 2007). It is a high-throughput technique with the ability to monitor changes in the
microbial communities’ structure and composition
(Schütte et al., 2008).