2.3. Extraction of the essential oil of carrot juice with different
treatments
The extraction of essential oil was carried out according to the
method with slight modifications (Diao, Hu, Zhang, & Xu, 2014;
Gao et al., 2011). The carrot juices with different treatments were
subjected to hydro distill for 6 h in a Clevenger-type apparatus. The
oily layer obtained on top of the aqueous distillate was separated
and dried with anhydrous sodium sulphate, then stored in a tightly
closed dark vial at 4 C until chemical composition analysis and
antimicrobial activity studies.