The pathogens were obtained from symptomatic cassava leaves in the following manner.
The conidia were gently washed from characteristic lesions with sterilized water, and the final concentration of the conidial suspensions was adjusted to 103 conidia/mL. Potatodextrose agar (PDA) medium plates containing 100 μg/mL ampicillin, kanamycin, and cephalothin were prepared. A 20-μL conidia solution was evenly coated on the surface andcultured
at 28°C for two days. When small colonies emerged, they were transferred to new PDA platesand cultured under the same conditions. The culture and morphological characteristics were observed 30 days later.