The skin samples obtained from the patients showed cell
morphology bearing the hyphae that resembled the shape
of spaghetti and meatball. The samples were tested positive
for catalase due to the presence of gas bubbles in reaction to
10% hydrogen peroxide (H2O2). Out of the five skin samples,
3 isolates were shown to be similar to the morphology of
Malassezia sp. The isolates were named M1, M2, and M3,
respectively, with M1 and M2 growing on SDA with olive
oil and M3 without olive oil. The first isolate (M1) showed
a creamy white and smooth colony, while the second (M2)
isolate showed a creamy pink and smooth colony, both on
SDA with olive oil. The third (M3) isolate showed a creamy
white and smooth colony on SDA without olive oil. All
isolates were then observed microscopically by Gram staining
using a microscope. The morphology of cells according to
isolates was presented in Figures 1–3. All isolates showed
budding in the cells with M1 (Figure 1) and M2 (Figure 2)
showing broad based budding while M3 (Figure 3) showing
narrow based budding. Tween assimilation test indicated a
positive growth on Tween 40, Tween 60, and Tween 80 for
M1 (Figure 4) and positive growth on all the Tween 20, Tween
40, Tween 60, and Tween 80 for M2 isolate (Figure 5). M3
isolate, however, showed negative growth for all the Tween
concentration as observed in Figure 6.