Potato (Solanum tuberosum L.) is one of the major vegetable crops that are grown world –wide
because of its economical importance. The main purpose of this study was to investigate the production of
virus free plantlets from meristem culture in two cultivars of potato. The specific objectives was to study
genotype (2 commercial cultivars of S. tuberosum, Marophona and Agria), substrate combination including 3
planting bed soil/sand/perlite (1:1:1), turb/perlite (1:1) and turb/rice hull/perlite (1:1:1), pot size with 19 cm (large)
and 13 cm (small) diameters and their interactions on number and total weight of minitubers. A single excised
meristem tip was carefully placed on the filter paper bridge of the culture tubes containing MS liquid and then
semisolid medium. The virus-free plantlets were transferred to pots with above mentioned substrates and pots.
Statistical analysis revealed that there are significant differences between potato cultivars, substrates, pots size
and substrate × pot size interaction effects (p