Around 0.1 g of fermented rice was transferred to an Erlenmeyer
flask (100 ml) with 50 mL of sterile double-distilled
water, shaken for 20 min, and 1 ml of this solution was resuspended
in sterile double-distilled water and aliquots were then
spread on nutrient agar plates composed of the following
(g/L): peptic digest of animal tissue, 5.0; beef extract, 1.5; yeast
extract, 1.5 and sodium chloride, 5.0 (pH 7.0). Twelve organisms
were isolated and the isolated organisms were screened
for amylase activity. The organisms were grown on nutrient
agar plate containing 1% soluble starch. After 24 h incubation
at 37 C, 1% iodine was poured on the starch agar plates. A
clear zone of hydrolysis around the colony indicates a positive
result.