Fig. 1 Culture establishment and regeneration: a Young inflorescences and b soft base of young inflorescence in leaf axil. c, d induction of callus cultures of aloe on culture medium with 2,4-D. e Microphotograph of callus cultures at multiplication stage and f differentiation of globular structures. g, h Microphotographs of cultures at different stages of culture regeneration. i A clump of in vitro regenerated plantlets of Aloe. j In vitro regenerated shoots of Aloe on medium with higher concentration of BAP. k Bottom of culture vessels showing roots in regenerated plantlets and l soil transferred plants of aloe produced through tissue culture