Chromosomal DNA of each strain was extracted by the
standard protocol (Sambrook et al. 1989), and primers were
designed based on genome sequence information of B.
glumae BGR1 (Lim et al. 2009). The tofI/tofR locus on
chromosomal DNA was amplified by PCR with KOD FX
DNA polymerase (Toyobo Life Science, Osaka, Japan)
using the primer set