Fully grown green or ripe tomatoes were
picked. 20g of fresh tomato pulp and peel
were homogenized separately in the
blender in the presence of 0.1M 25mL
Sodium Phosphate buffer(pH6.5) each. The
homogenate was filtered through a filter
paper. The filtrates (29mL)were combined
and centrifuged at 30000g for 20 minute at
4C. The supernatant, to be referred to as
crude soluble PPO fraction, was stored to
further use.