Data are expressed as Mean 7 SD. Statistical differences between groups were determined by applying an unpaired Student's t-test when only two groups were compared. When more than 2 groups were compared a one-way ANOVA test was used fol- lowed by Tukey-Kramer Multiple Comparisons test. Statistical significance was set at P o 0.05. Using the GraphPad InStant Software Vers 3.05.