quantified by GLC as described previously (27). Fatty acids in subcutaneous
adipose tissue reflect long-term intake (29,30) and are
preferred in case-control studies over other biomarkers of fatty acid
intake (31). In the Costa Rican population, adipose tissue linoleic
acid, -linolenic acid, and trans fatty acids were found to be excellent
biomarkers of intake (22,27). We used cis- and trans-PUFAs in
adipose tissue to identify and confirm the presence of fatty acids that
are likely to be responsible for the observed results.