The method reported by Wang et al. [25] shows an HRM peak pattern with 2 peaks, and identification of species are based on a combination of each peak. Consequently, each Listeria spp. has multiple patterns. In the method established in the present study, there was generally 1 peak pattern per species of Listeria, and it has advantages of simple waveform determination. A subsequent analysis using ldh is necessary only where a strain is thought to be L. monocytogenes or L. welshimeri, and since it only requires the analysis of Tm values, it is simple to perform.