sign-rank test. The probability distribution was established using 1000
simulations under three models–Infinite allele model (IAM), stepwise
mutation model (SMM) and two-phase model of mutation (TPM). The
second method was the graphical representation of the mode-shift
indicator originally proposed by Luikart et al. (1998). Loss of rare alleles
in bottlenecked populations is detected when one or more of the common
allele classes have a higher number of alleles than the rare allele
class (Luikart et al., 1998). These two methods were applied using Bottleneck
v1.2.02