The present study is focused on the development of a cultivation-independent molecular approach for specific detection of bioluminescent bacteria within microbial communities by direct amplification of luxA gene from environmental DNA
A new set of primers ,specifically targeting free-living bioluminescent bacteria , was designed on the base of luxA sequences available from the public database
Meso - and bathypelagic seawater samples were collected from two stations in TyrrhenianSea at the depths of 500 and 2750 m.