This work describes a reproducible and accurate on-line SPE HPLC with UV absorbance detection method for determining carbendazim in orange juice. This application was performed using an UltiMate 3000 x2 Dual RSLC system controlled by Chromeleon CDS software version 7.1. A salting-out extraction combined with on-line SPE simplified the extraction and improved HPLC detection sensitivity. The measured MDL of carbendazim in orange juice was 0.2 µg/L.