A commercial dry baker’s yeast Saccharomyces cerevisiae was
purchased from a local store and dispersed in sterile water at room
temperature at a concentration of 10 g/L (g dry baker’s yeast/liter
of distilled water). The yeast was incubated on agar plates
containing 20 g/L agar, 20 g/L glucose, 10 g/L yeast extract and
20 g/L peptone for 2 days at 30 C. One loop from the agar plate
was transferred into liquid YPD medium where it was precultivated
for 24 h at 30 C and 150 rpm in a shaking incubator.
Fermentation medium contained: 20 g/L glucose, 10 g/L yeast
extract, 10 g/L peptone, 3 g/L Na2HPO4 7H2O, 3 g/L KH2PO4,
0.25 g/L MgSO4 7H2O, 0.08 g/LCaCl22H2O. The precultivated cultures
were finally transferred to the fermentation media at an
inoculum size of 10% (v/v) for ethanol production.
A commercial dry baker’s yeast Saccharomyces cerevisiae waspurchased from a local store and dispersed in sterile water at roomtemperature at a concentration of 10 g/L (g dry baker’s yeast/literof distilled water). The yeast was incubated on agar platescontaining 20 g/L agar, 20 g/L glucose, 10 g/L yeast extract and20 g/L peptone for 2 days at 30 C. One loop from the agar platewas transferred into liquid YPD medium where it was precultivatedfor 24 h at 30 C and 150 rpm in a shaking incubator.Fermentation medium contained: 20 g/L glucose, 10 g/L yeastextract, 10 g/L peptone, 3 g/L Na2HPO4 7H2O, 3 g/L KH2PO4,0.25 g/L MgSO4 7H2O, 0.08 g/LCaCl22H2O. The precultivated cultureswere finally transferred to the fermentation media at aninoculum size of 10% (v/v) for ethanol production.
การแปล กรุณารอสักครู่..
