An amount of
0.5 g of biomass and 0.5 g of diatomaceous earth was
macerated for 10 min using a pestle in a mortar, to which
6 mL of acetone were added. (3) Abrasion with glass
pearls: This technique was performed by adapting the
method proposed by Medeiros et al. (18). Tubes containing
0.5 g of biomass and 6 mL of acetone were added with a
load of 1.1 g/mL glass pearls (0.5-0.59 mm) and agitated
vigorously for 10 min in vortex agitator. (4) Immersion in
liquid nitrogen: Tubes containing 0.5 g of biomass were
added with 6 mL of acetone, followed by immersion in
liquid nitrogen and subsequent maceration of the frozen
mixture using pestle in a mortar for 1 min. After applying
the techniques of cell disruption, the supernatant was
separated by centrifugation (1,745×g for 10 min) for
subsequent extraction of carotenoids.