The alignment of nanofibers was assesses by measuring the angle of fiber from SEM images (n = 50 at each condition). The degree of alignment was determined as a reference angle (zero angle) and all deviations were measured relative to that angle. After that, these values were then normalized to 90 degrees and plotted in a histogram. If the values are closer to 90 degrees, it will indicate more alignment. The histogram demonstrates the alignment of fiber as a function of frequency.