and initial sugar concentration was adjusted to 25 Brix. For sterilization, 200 ppm of
potassium metabisulfite (K2S2O5) was added and placed for 2 h at
room temperature. For alcohol fermentation, 1 g/L of dry yeast (S.
cerevisiae) was added and the mixture was incubated at 27 C for 6
days. When the alcoholic fermentation was completed, the intermixture was centrifuged (350 g, 10 min) and the supernatant was
kept in a 70 C water bath for 30 min. Each batch was stopped when
the alcohol content reached 15% (w/v).