The leaves powder samples were used for the extraction with
methanol by maceration method. After complete extraction,
methanol was evaporated by rotary evaporator and defatted
with water. Different solvents such as hexane, chloroform,
ethyl acetate and butanol were used for extraction with
increasing polarities.
3.1. Total phenolics content
All crude extracts from the leaves of neem were used for the
determination of total phenolics content by using Foline
Ciocalteu reagent (Hossain et al., 2013). The results of total
phenolics content by using FolineCiocalteu reagent are presented
in Table 1. From Table 1, the highest amount of total
phenolics was obtained in butanol crude extract and the
lowest was chloroform crude extract. The order of total phenolics
was butanol > ethyl acetate > water > methanol
> hexane > chloroform. The gallic acid was used for a standard
calibration curve (Table 1 and Fig. 2).