Resultsanddiscussion
3.1. Characterization by GC–MS.Quantification and semi-quantification byGC-FID and statistical results
GC–MS analysis of the essential oil resulting from HD,SDE and “commercial distillation” provided the relative percentage(%of total essential oil) for each compound present in the volatile profile.
Table 2 shows the families of volatile compounds identified in the essential oils of the studied Lamiaceae plants and the commercial essential oil samples, along with the irretention time.
A GC-FID method was developed for determining and quantifying carvacrol, thymol and pulegone present in essential oils extracted only by HD technique, as it gave higher yields,suitable for quantitative analysis.Calibration curves were obtained by analyzing six standard solutions containing increasing concentrations of the respective standard and covering the range of linearity
(carvacrol: 0.05–15g/L,thymol:0.05–25g/Land(R)-(+)-pulegone:
0.05–20 g/L).The solutions were prepared in diethyl ether.
Resultsanddiscussion3.1. Characterization by GC–MS.Quantification and semi-quantification byGC-FID and statistical resultsGC–MS analysis of the essential oil resulting from HD,SDE and “commercial distillation” provided the relative percentage(%of total essential oil) for each compound present in the volatile profile.Table 2 shows the families of volatile compounds identified in the essential oils of the studied Lamiaceae plants and the commercial essential oil samples, along with the irretention time.A GC-FID method was developed for determining and quantifying carvacrol, thymol and pulegone present in essential oils extracted only by HD technique, as it gave higher yields,suitable for quantitative analysis.Calibration curves were obtained by analyzing six standard solutions containing increasing concentrations of the respective standard and covering the range of linearity(carvacrol: 0.05–15g/L,thymol:0.05–25g/Land(R)-(+)-pulegone:0.05–20 g/L).The solutions were prepared in diethyl ether.
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