Delimitation of a candidate genomic region of BPH26
High-resolution genetic maps of BPH26 were constructed using 5,349 BC6F3 individuals (Fig. 1a). Seventy-nine plants showing recombination between two markers (RM28449 and RM3813) were selected, and the BPH resistance of these plants was analyzed based on the survival rate of BPH in a test tube. Eventually, we determined the candidate genomic region of BPH26 in a 135-kb region between two flanking markers, DS-72B4 and DS-173B (Fig. 1a).