The use of silk fiber in the field of medical is known from centuries as suture. [Lee et al.2004] The range of applications of silk fiber is expanding day by day in the field of medical as biomaterials due to its thermal stability, biodegradability and biocompatibility. Silk proteins need to be engineered into useful forms for proper applications in the field of biomedical such as suture, biosensors, scaffold for tissue engineering and wound dressing materials. The properties of silk fiber can be tailor-made for its suitable use in medical textiles either by surface modification or by its regeneration in the form of films or fibers. The regeneration of silk fibers by wet-spinning and electrospinning process have various capabilities, such as controlling the structure and properties of silk fiber, allowing silk fiber to be blended with other polymers, immobilizing the biological materials etc. The regeneration of silk fibers is carried out in three steps, namely degumming, fibroin solution preparation and fiber spinning. Fig. 1 shows the line diagram of the techniques involved in the regeneration process of silk in the form of fibers and films.