The fatty acid methyl esters (FAME) profile was determined using a capillary gas chromatograph
equipped with a 100 m 0.25 mm 0.2 µm column (Supelco
SP 2560 (Metlab, Deeside, UK) split ratio 50:1, injector
temperature 250 ° C) and a flame ionisation detector (hydrogen
30 ml/minute, air 400 ml/minute, temperature 260 ° C) with helium
used as a carrier gas (43.51 PSI at 170 ° C).