Samples collection of L. japonica and G. lemaneiformis were from September 2012 to December 2012 and from January 2013 to May 2013 monthly, respectively. After the collection, samples were rinsed and placed in oxygenated sealed plastic boxes filled with local seawater, kept under cool conditions, and transported to the laboratory. In the laboratory, samples were rinsed gently with running filtered seawater (0.45 mm filter membrane), rinsed with distilled water 3–4 times