Intracellular and extracellular oxidative stress initiated by reactive oxygen species (ROS) advance skin aging, which is characterized by wrinkles and atypical pigmentation. Skin aging also is usually discussed in relation to UV exposure. The objectives of this study were to investigate the antioxidant activity and antiaging effect of carrot glycoprotein (CG), it is compared with the fish scale collagen peptide (SCP) and investigate the effects on the nutritional quality (carotenoid). As a result carrot glycoprotein expressed a similar content of total flavonoid compared with collagen peptide and expressed comparatively higher electronic donation activity than collagen peptide. Also have higher inhibition effect of lipid peroxidation generally than a comparison group (BHA and vitamin E). In vitro the cytotoxic experiment, the toxicity didn’t appear in the concentration less than CG 1.0 mg/ml. CG promoted the generation of collagen type-1 in more than 0.5 mg/ml concentration and SCP did so in more than 0.3 mg/ml concentration. CG was reduced MMP-1 meaningfully and statistically all in the concentration of 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml, and SCP showed the inhibition of MMP-1 expression in 0.3 mg/ml. These results express that CG can be applied to new material related to antioxidants and antiaging.