and RiTTeR (1994). Folin-Ciocalteu reagent (75 μL) was mixed with 15 μL of extract and 1260 μL of distilled water. After 3 min, 150 μL of a saturated Na2CO3 solution were added. After 1 h, the absorbance of the sample was measured at 720 nm. The TPP was calculated from a calibration curve obtained using caffeic acid and thus was expressed as caffeic acid equivalents. The antioxidant capacity of the extract was determined using the method of kao and chen (2006) with modifications. An aqueous solution of 7 mM ABTS was prepared, to which 2.45 mM potassium persulphate were added to generate the ABTS radical (ABTS•+) by incubation in the dark at ambient temperature for 12 h.