Effects of temperature on H2 productivity of strain AM2
To determine the optimum temperature for H2 production, H2 production rates of strain AM2 were measured at pH 6 with a temperature range of 25 °C–42 °C (Fig. 1(C)). The results showed that the H2production rates were strongly dependent on the temperature of the culture; the maximum production rate of H2 was observed at 37 °C in both substrate-grown cells, with production rates of 9.5 and 8.0 mmol/h/L from glucose and mannitol, respectively (Fig. 1(C)). The maximum production rates of H2 at 37 °C from glucose and mannitol were 1.5 and 1.2 times higher than those at 30 °C, respectively. The H2 production rates from glucose were higher than those from mannitol at all temperatures. At 25 °C, the difference between glucose and mannitol was the largest. In the culture from glucose at 42 °C, the volume of H2production was lower than at 25 °C, and H2 production stopped after 34.5 h (data not shown). Finally, at pH 6 and 37 °C, the molar yield of H2 of strain AM2 reached 1.5 mol H2/mol glucose and 1.7 mol H2/mol mannitol in batch culture.
Effects of temperature on H2 productivity of strain AM2
To determine the optimum temperature for H2 production, H2 production rates of strain AM2 were measured at pH 6 with a temperature range of 25 °C–42 °C (Fig. 1(C)). The results showed that the H2production rates were strongly dependent on the temperature of the culture; the maximum production rate of H2 was observed at 37 °C in both substrate-grown cells, with production rates of 9.5 and 8.0 mmol/h/L from glucose and mannitol, respectively (Fig. 1(C)). The maximum production rates of H2 at 37 °C from glucose and mannitol were 1.5 and 1.2 times higher than those at 30 °C, respectively. The H2 production rates from glucose were higher than those from mannitol at all temperatures. At 25 °C, the difference between glucose and mannitol was the largest. In the culture from glucose at 42 °C, the volume of H2production was lower than at 25 °C, and H2 production stopped after 34.5 h (data not shown). Finally, at pH 6 and 37 °C, the molar yield of H2 of strain AM2 reached 1.5 mol H2/mol glucose and 1.7 mol H2/mol mannitol in batch culture.
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