3. Result
The locus A781G in exon 2 of the GH gene (Accession No-DQ461672.1), was amplified from ovine genomic DNA. The size of amplicon obtained was 422 bp. The 422 bp fragmentincludes parts of exon 2 (126 bp), intron 2 (227 bp) and aportion of exon 3 (69 bp) of GH gene. Restriction diges-tion of the amplified region by the endonuclease, HaeIII with a recognition sequence – GG/CC, showed polymor-phism and revealed the existence of two genotypes viz.,AA (366 bp and 56 bp) and AB (422 bp, 366 bp and 56 bp),characterized by the presence of two alleles namely A and B (Fig. 1). BB genotype was absent. Genotypic frequen-cies for AA and AB were 0.43 and 0.57, respectively. Theallele frequencies were 0.71 and 0.29 for A and B alleles respectively. This variation is due to A781G transition,which caused an amino acid change from Serine to Glycine.The highly significant (P < 0.001) Chi-square value (17.91)showed that the population is not under Hardy–Weinberg equilibrium.
3. ResultThe locus A781G in exon 2 of the GH gene (Accession No-DQ461672.1), was amplified from ovine genomic DNA. The size of amplicon obtained was 422 bp. The 422 bp fragmentincludes parts of exon 2 (126 bp), intron 2 (227 bp) and aportion of exon 3 (69 bp) of GH gene. Restriction diges-tion of the amplified region by the endonuclease, HaeIII with a recognition sequence – GG/CC, showed polymor-phism and revealed the existence of two genotypes viz.,AA (366 bp and 56 bp) and AB (422 bp, 366 bp and 56 bp),characterized by the presence of two alleles namely A and B (Fig. 1). BB genotype was absent. Genotypic frequen-cies for AA and AB were 0.43 and 0.57, respectively. Theallele frequencies were 0.71 and 0.29 for A and B alleles respectively. This variation is due to A781G transition,which caused an amino acid change from Serine to Glycine.The highly significant (P < 0.001) Chi-square value (17.91)showed that the population is not under Hardy–Weinberg equilibrium.
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