Before (in the evening before sleep) and after (in the morning) applying topical cream, all subject should wash his/her face with soft soap which was also provided. Blood specimens For evaluating the levels of MDA we randomized 20 subjects in each group, calculated based on previous study by Surlinia.8 After randomization of the subjects, 2 subjects in control group were dropped out, hence there were 18 subjects in control group and 20 subjects in treatment group whose blood specimen were taken