Samples were also examined with Confocal Laser Scanning
Microscopy to reveal the protein and oil distribution. The particle
dispersions were diluted 5 times, stained with a few drops of Nile
Blue (Sigma–Aldrich, St. Louis, MO, USA) and directly analysed by
CSLM. Nile Blue is a fluorescent dye suitable to monitor both the
protein and the oil distribution in the sample (Sag˘lam et al.,
2011). Samples were excited at 488 nm and measured at 520–
602 nm to detect the oil signal,