Amylose can be divided into long-chain amylose (LCAM) and shortchain amylose (SCAM) based on the gel-permeation chromatography (Annor et al., 2014b). The ratio of LCAM:SCAM (weight-based) of four millet genotypes ranged from 0.6 to 1.3. Based on the gel-permeation chromatography and iodine binding properties of the β-LDs of whole starch, structural difference in the amylose branching pattern was suggested.