Improving GABA production through changing the soaking
solution has been studied as well. Oh and Choi (2000) reported that
BR germinated for 72 h with immersion in chitosan solution
(100 ppm) had 7.6 times more GABA than that in native BR, and
1.3 times more than that in the control germinated in distilled
water. Moreover, chitosan was effective in suppressing mould
growth during immersion and germination. Under the same conditions,
glutamic acid solution for immersion likewise demonstrated
8.8 and 1.2 times more GABA than those in un-germinated and in
the control treated in distilled water, respectively (Oh, 2003). Lu
et al. (2010) reported that electrolyzed oxidizing water resulted
in shortened sprouts and radicles but increased GABA content to
nearly double the amount in the control GBR. In addition, the electrolyzed
oxidizing water was highly effective in inhibiting microbial
growth during germination. The effects of cultivar and
germination method on GABA content in GBR are summarized in