A. calamus rhizome and leaves are traditionally used for the treatment of various neurological disorders due to its neuro-protective properties. Hydro-alcoholic extract of A. calamus has demonstrated neuroprotective effects in the middle cerebral arteryocclusion-induced ischemia in rats (Shukla et al. 2006). α and β-Asarone inhibited N-Methyl-d-Aspartate (NMDA) and glutamateinduced excitotoxicity in primary cortical cultures (Cho et al. 2002) Studies by Sandeep and Nair (2010) have demonstrated in vitroradio-protective effects of A. calamus. The protective effects were evaluated by measuring the degree of lipid peroxidation. In vitro DNA damage was measured by assessing the radiation induced relaxation of super coiled plasmid DNA (pBR322) and alkaline sin-gle cell gel electrophoresis or comet assay was used to analyze any damage to cellular DNA. The free radical scavenging property of A.calamus safeguarded DNA and membrane damage caused due to -radiations in murine cells and human peripheral blood leukocytes