The effect of addition of micronutrients (Stock C) could be
determined when the explant cultured in Media D was compared
with those in control media (Media B). According to Fig. 3, the
growth index of H. callitrichoides in Media D was 4.02
0.05 g/g
after
fifteen days into the cultivation. The addition of micronutrients
to 1/2 strength POME did not give any significant effect to
the growth index of H. callitrichoides (P > 0.05). The chlorotic
characteristics of leaves in Media B and D was mainly contributed
by the deficiency in macronutrients mentioned above [21].