This study was conducted to evaluate the response of ash (Fraxinus excelsior) seeds under accelerated aging test. The accelerated aging test was carried out at three different temperatures: 41, 43 and 45°C with four duration periods of 48, 72, 96, 144 and a relative humidity of 100%. The two seed lots of F. excelsior were subjected to tests of their quality including standard germination, vigor index, seedling growth and seedling dry weight. A completely randomized factorial design with four replications was used. Results indicated that, old seeds were more sensitive to accelerated aging than new seeds. Seeds of both lots had a reduction of viability and vigor after accelerated aging at 41°C for 96 h, 43 and 45°C for 72 to 144 h. Germination, vigor index, seedling growth and seedling dry weight showed a greater response to accelerated aging.