Random mutagenesis and RNA-seq selected many potential regulators. The specific mutation provided preliminarily evidence regarding several toxoflavin regulators that compensate in the absence of tofI or tofR. Complementation tests need to be done in order to confirm their roles in toxoflavin production. Mechanisms of important
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regulators should be approached by different post-translational tests in the future to build upon the knowledge of the entire signaling system in B. glumae. For the QS dependent gene candidates selected from RNA-seq, more genetic and protein level work will need to be done before any conclusions can be drawn about their true function and targets. So far, the transcriptome analysis results in this dissertation provide some potential direction in future research areas.