Both white and gold sesame seed powders (100 g) were defatted
using acetone as a solvent in which fats were dissolved. Briefly,
acetone was added (1:4, w/v) to the sesame powder, which was
extracted for 24 h at room temperature. Next, acetone–sesame
powder mixture was filtered and the residue was washed with
diethyl ether. The obtained dried defatted powder was extracted
with 70% ethanol (1:10, w/v) for 1 h at room temperature; the
extraction was performed three times to obtain a better yield.
The polar-soluble extract obtained was concentrated by ethanol
evaporation using a rotary evaporator and remaining aqueous
solution was lyophilized to obtain white or gold sesame polar-soluble
crude extract powder.